A comparison of alternative assays to measure DNA damage in stallion spermatozoa: TUNEL test versus ‘Nicoletti assay’
نویسندگان
چکیده
منابع مشابه
Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay.
BACKGROUND In this study we present the physiological changes observed in ejaculated spermatozoa of normospermic men after exposure to hydrogen peroxide (H(2)O(2)) or gamma irradiation. METHODS Motility changes as well as membrane and DNA-damage were determined in spermatozoa after incubation with 25 micromol/l of H(2)O(2) during increasing intervals of time (0--60 min and after 24 h) or afte...
متن کاملEvaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality
Sperm DNA damage affects the conception rate resulting from human assisted reproduction technology. The objective of this study was to adapt the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay to provide a quality parameter for bull semen based on the detection of sperm DNA damage. Fresh semen was collected from two Japanese Black bulls (A, B) several times o...
متن کاملTUNEL as a test for sperm DNA damage in the evaluation of male infertility.
OBJECTIVES To standardize the TUNEL assay by establishing inter- and intraobserver variability, interassay variability, cutoff values, sensitivity and specificity of the assay, and studying the distribution of the DNA damage in a population of infertile men referred to a clinical andrology laboratory. METHODS Seminal ejaculates from 25 healthy male volunteers (controls) and 194 infertile men ...
متن کاملAnalysis of chromatin integrity and DNA damage of buffalo spermatozoa
This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...
متن کاملToluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays.
BACKGROUND Sperm DNA integrity (SDI) is an important factor in the prognosis of male fertility. Here we compare the toluidine blue (TB) image cytometry test, recently proposed by us for SDI assessment, with two other tests-the sperm chromatin structure assay (SCSA) and the terminal nick-end labelling (TUNEL) assay. METHODS Sperm samples from 35 men were evaluated for standard sperm parameters...
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ژورنال
عنوان ژورنال: Acta Veterinaria Hungarica
سال: 2015
ISSN: 0236-6290,1588-2705
DOI: 10.1556/avet.2015.010